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61.
Mehdi Forouzesh Adil Askerovich Mamedov Amirasad Pourabadeh Mojgan Hosseini 《Journal of biomolecular structure & dynamics》2020,38(12):3750-3756
Abbreviations COM center of mass distance MD molecular dynamics MM-PBSA Molecular Mechanics Poisson–Boltzmann Surface Area Nb nanobody PlGF placenta growth factor Rg radius of gyration RMSD root mean-square deviation SASA solvent-accessible surface area VEGF vascular endothelial growth factor 相似文献
62.
Beatrice Carlsson Elin Kindberg Javier Buesa Gustaf E. Rydell Marta Fos Lidón Rebeca Montava Reem Abu Mallouh Ammi Grahn Jesús Rodríguez-Díaz Juan Bellido Alberto Arnedo G?ran Larson Lennart Svensson 《PloS one》2009,4(5)
In November 2004, 116 individuals in an elderly nursing home in El Grao de Castellón, Spain were symptomatically infected with genogroup II.4 (GII.4) norovirus. The global attack rate was 54.2%. Genotyping of 34 symptomatic individuals regarding the FUT2 gene revealed that one patient was, surprisingly, a non-secretor, hence indicating secretor-independent infection. Lewis genotyping revealed that Lewis-positive and negative individuals were susceptible to symptomatic norovirus infection indicating that Lewis status did not predict susceptibility. Saliva based ELISA assays were used to determine binding of the outbreak virus to saliva samples. Saliva from a secretor-negative individual bound the authentic outbreak GII.4 Valencia/2004/Es virus, but did not in contrast to secretor-positive saliva bind VLP of other strains including the GII.4 Dijon strain. Amino acid comparison of antigenic A and B sites located on the external loops of the P2 domain revealed distinct differences between the Valencia/2004/Es and Dijon strains. All three aa in each antigenic site as well as 10/11 recently identified evolutionary hot spots, were unique in the Valencia/2004/Es strain compared to the Dijon strain. To the best of our knowledge, this is the first example of symptomatic GII.4 norovirus infection of a Lea+b− individual homozygous for the G428A nonsense mutation in FUT2. Taken together, our study provides new insights into the host genetic susceptibility to norovirus infections and evolution of the globally dominating GII.4 viruses. 相似文献
63.
All crocodilians are under varying degrees of threat due to over exploitation and these species have been listed in Appendix
I or II of CITES. The lack of molecular techniques for the identification of confiscated samples makes it difficult to enforce
the law. Conclusive forensic identification of species requires a complete gene sequence which is difficult in case of degraded
samples. We have developed two novel sets of primers to amplify two partial cytochrome b gene sequences of six crocodile species i.e. Crocodylus palustris, Crocodylus porosus, Crocodylus siamensis, Crocodylus niloticus, Gavialis gangeticus and Caiman crocodilus. These partial sequences were edited to give a complete cyt b gene sequence, which can be used as an effective tool for forensic authentication of crocodile species. A phylogeny of crocodile
species was reconstructed using these sequences. The described primers hold great promise in forensic identification of crocodile
species, which can aid in the effective enforcement of law and conservation of these ancient species. 相似文献
64.
Species identification has been the core issue in all approaches of conservation of endangered wild life. In this regard molecular
techniques for species authentication have proved indispensable. A novel multiplex PCR assay for the identification of three
Indian snake species Python morulus, Ptyas mucosus, and Naja naja is successfully demonstrated using 16S rRNA gene. Three reverse primers and a common forward primer were designed to generate
three different size species-specific PCR fragments. Absence of any PCR amplification in non-target species proves the specificity
of the primers. These four primers were combined in a multiplex assay to enable identification of three snake species in a
single reaction. The assay described here shows its utility in identifying unknown snake specimen and in case of samples yielding
low quality DNA. This multiplex PCR technique using novel primers is an unprecedented approach offered for forensic identification
of exhibits originating from three Indian snake species. It is expected that this endeavor will help strengthening conservation
efforts for these species. 相似文献
65.
Christopher T. Price Souhaila Al-Khodor Tasneem Al-Quadan Marina Santic Fabien Habyarimana Awdhesh Kalia Yousef Abu Kwaik 《PLoS pathogens》2009,5(12)
The ability of Legionella pneumophila to proliferate within various protozoa in the aquatic environment and in macrophages indicates a remarkable evolution and microbial exploitation of evolutionarily conserved eukaryotic processes. Ankyrin B (AnkB) of L. pneumophila is a non-canonical F-box-containing protein, and is the only known Dot/Icm-translocated effector of L. pneumophila essential for intra-vacuolar proliferation within both macrophages and protozoan hosts. We show that the F-box domain of AnkB and the 9L10P conserved residues are essential for intracellular bacterial proliferation and for rapid acquisition of polyubiquitinated proteins by the Legionella-containing vacuole (LCV) within macrophages, Dictyostelium discoideum, and Acanthamoeba. Interestingly, translocation of AnkB and recruitment of polyubiquitinated proteins in macrophages and Acanthamoeba is rapidly triggered by extracellular bacteria within 5 min of bacterial attachment. Ectopically expressed AnkB within mammalian cells is localized to the periphery of the cell where it co-localizes with host SKP1 and recruits polyubiquitinated proteins, which results in restoration of intracellular growth to the ankB mutant similar to the parental strain. While an ectopically expressed AnkB-9L10P/AA variant is localized to the cell periphery, it does not recruit polyubiquitinated proteins and fails to trans-rescue the ankB mutant intracellular growth defect. Direct in vivo interaction of AnkB but not the AnkB-9L10P/AA variant with the host SKP1 is demonstrated. Importantly, RNAi-mediated silencing of expression of SKP1 renders the cells non-permissive for intracellular proliferation of L. pneumophila. The role of AnkB in exploitation of the polyubiquitination machinery is essential for intrapulmonary bacterial proliferation in the mouse model of Legionnaires'' disease. Therefore, AnkB exhibits a novel molecular and functional mimicry of eukaryotic F-box proteins that exploits conserved polyubiquitination machinery for intracellular proliferation within evolutionarily distant hosts. 相似文献
66.
Abu Hena Mostafa Kamal Ki-Hyun Kim Kwang-Hyun Shin Hyung-Seok Seo Hisashi Tsujimoto Hwa-Young Heo Jong-Soon Choi Chul-Soo Park Sun-Hee Woo 《Journal of Plant Biology》2009,52(6):533-542
Glutenin is a major determinant of baking performance and viscoelasticity, which are responsible for high-quality bread with
a light porous crumb structure of a well-leavened loaf. We analyzed the diversity of glutenin genes from six wheat cultivars
(Korean cvs. Keumgang and Jinpum, Chinese cvs. China-108 and Yeonnon-78, and Japanese cvs. Norin-61 and Kantou-107). Glutenins
contain two types of isoforms such as high molecular weight glutenin subunit (HMW-GS) and low molecular weight glutenin subunit
(LMW-GS). Glutenin fractions were extracted from wheat endosperm using Osborne solubility method. A total of 217 protein spots
were separated on two-dimensional gel electrophoresis with isoelectric focusing (wide range of pH 3–10). The proteins spots
were subjected to tryptic digestion and identified by matrix assisted laser desorption/ionization–time of flight mass spectrometry.
HMW-GS (43 isoforms) and LMW-GS (seven isoforms) are directly responsible for producing high-quality bread and noodles. Likewise,
all the seed storage proteins are digested to provide nutrients for the embryo during seed germination and seedling growth.
We identified the diverse glutenin subunits in wheat cultivars and compared the gluten isoforms among different wheat cultivars
according to quality. This work gives an insight on the quality improvement in wheat crop. 相似文献
67.
Maren de Vries Adil S. Mohamed Rachel A. Prescott Ana M. Valero-Jimenez Ludovic Desvignes Rebecca OConnor Claire Steppan Joseph C. Devlin Ellie Ivanova Alberto Herrera Austin Schinlever Paige Loose Kelly Ruggles Sergei B. Koralov Annaliesa S. Anderson Joseph Binder Meike Dittmann 《Journal of virology》2021,95(10)
68.
Md. Rezwan Molla Md. Motiar Rohman Mahmuda Binte Monsur Mirza Hasanuzzaman Lutful Hassan 《Phyton》2021,90(5):1425-1443
This study was undertaken to investigate oxidative stress tolerant mechanisms in chilli (Capsicum annuum L.)
under drought genotypes through evaluating morphological, physiological, biochemical and stomatal parameters.
Twenty genotypes were evaluated for their genetic potential to drought stress tolerant at seedling stage. Thirty
days old seedlings were exposed to drought stress induced by stop watering for the following 10 days and rewatering for the following one week as recovery. Based on their survival performance, two tolerant genotypes
viz. BD-10906 and BD-109012 and two susceptible genotypes viz. BD-10902 and RT-20 were selected for studying
the oxidative stress tolerance mechanism. Drought reduced root and shoot length, dry weight, ratio, petiole weight
and leaf area in both tolerant and susceptible genotypes, and a higher reduction was observed in susceptible genotypes. Lower reduction of leaf area and photosynthetic pigments were also found in tolerant genotypes. Moreover,
tolerant genotypes showed higher recovery than susceptible genotypes after the removal of stress. A higher reduction
of relative water content (RWC) may cause an imbalance between absorbed and transpirated water in susceptible
genotypes. Higher accumulation of proline in tolerant genotypes might be helpful to for better osmotic maintenance
than that in susceptible genotypes. Tolerant genotypes showed higher antioxidant activity as they showed DPPH
radical scavenging percentage than the susceptible genotypes. Moreover, closer stomata in tolerant genotypes than
susceptible ones helped to avoid dehydration in tolerant genotypes. Thus, the above morphological, physiological,
biochemical and stomatal parameters helped to show better tolerance in chilli under drought stress. 相似文献
69.
Sukham Munilkumar Yumnam Rameshori W. Romen Mangang Makamguang Kamei Yengkhom Chinglemba Annam Pavan-Kumar Bijay Kali Mahapatra Arabinda Das Md. Abu Hanif Muhammad Abu Bakar Siddik 《Zeitschrift fur angewandte Ichthyologie》2021,37(2):356-358
The length-weight relationships (LWRs) of six Nemacheilid species (Schistura chindwinica, S. fasciata, S. khugae, S. minuta, S. reticulata and S. rubrimaculata) have been analyzed. Fish samples were collected on quarterly basis from March 2018 to February 2019. Sampling was performed using cast nets (mesh size 5–10 mm; about 50 sq m area covered each time and water depth was 4 ft approx.), and electrofishing (Ultrasonic Inverter Electro Fisher, 24 volts, 4 m) in the day time. The total length (TL) of individual fish was measured to 0.1 cm with a digital caliper and body weights (BW) were measured to 0.001 g with digital electronic balances. The parameters for the LWR equations were calculated, and the respective statistics such as the 95% confidence interval for parameters “a” and “b” are provided as well as the coefficient of correlation. For five species a new maximum total length has been documented. 相似文献
70.
Baruah Indrani Baruah Geetanjali Sahu Jagajjit Singha Dhanawantari L. Dekaboruah Hariprasanna Velmurugan Natarajan Chikkaputtaiah Channakeshavaiah 《Plant Molecular Biology Reporter》2021,39(2):471-471
Plant Molecular Biology Reporter - The affiliation 2 in the published article was Academy of Scientific and Innovative Research (AcSIR), CSIR-NEIST Campus, Jorhat, Assam 785,006, India. 相似文献